RELION
From ScientificComputing
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Category
BioinformaticsDescription
RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) JSB.Available versions (Euler, old software stack)
Legacy versions | Supported versions | New versions |
---|---|---|
1.3, 2.0.3, 2.1.b1, 3.0.0_beta |
Environment modules (Euler, old software stack)
Version | Module load command | Additional modules loaded automatically |
---|---|---|
1.3 | module load new gcc/4.8.2 open_mpi/1.6.5 relion/1.3 | |
2.0.3 | module load new gcc/4.8.2 open_mpi/1.6.5 fftw/3.3.3 relion/2.0.3 | |
2.1.b1 | module load new gcc/4.8.2 open_mpi/1.6.5 fftw/3.3.3 relion/2.1.b1 | |
3.0.0_beta | module load new gcc/4.8.2 open_mpi/1.6.5 relion/3.0.0_beta |
License information
GPLv2Links
http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Pagehttps://github.com/jjcorreao/relion/tree/master/relion-1.3
http://www2.mrc-lmb.cam.ac.uk/groups/scheres/relion13_tutorial.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318617
http://www.sciencedirect.com/science/article/pii/S1047847712002481