RELION

Category

Bioinformatics

Description

RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) JSB.

Available versions

Legacy versions	Supported versions	New versions
		1.3, 2.0.3, 2.1.b1, 3.0.0_beta

Environment modules

Version	Module load command	Additional modules loaded automatically
1.3	module load new gcc/4.8.2 open_mpi/1.6.5 relion/1.3
2.0.3	module load new gcc/4.8.2 open_mpi/1.6.5 fftw/3.3.3 relion/2.0.3
2.1.b1	module load new gcc/4.8.2 open_mpi/1.6.5 fftw/3.3.3 relion/2.1.b1
3.0.0_beta	module load new gcc/4.8.2 open_mpi/1.6.5 relion/3.0.0_beta

License information

GPLv2

Links

http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page

https://github.com/jjcorreao/relion/tree/master/relion-1.3
http://www2.mrc-lmb.cam.ac.uk/groups/scheres/relion13_tutorial.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318617
http://www.sciencedirect.com/science/article/pii/S1047847712002481